2023

l Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1.Liu Yi-Shi, Wang Yicheng, Zhou Xiaoman, Zhang Linpei, Yang Ganglong, Gao Xiao-Dong, Murakami Yoshiko, Fujita Morihisa, Kinoshita Taroh. Journal of Cell Biology, 2023, 222(5): e202208159.https://doi.org/10.1083/jcb.202208159

l A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.Jia Ji-Xiang, Peng Sen-Lin, Kalisa Ndayambaje Yvan, Chao Qiang, Zhou Zhifang, Gao Xiao-Dong, Wang Ning. Journal of Nanobiotechnology, 2023, 21(1): 1-12.https://doi.org/10.1186/s12951-023-01927-x

l Efficient enzymatic synthesis of d‐allulose using a novel d‐allulose‐3‐epimerase from Caballeronia insecticola.Li Zijie, Feng Linxue, Chen Zhou, Hu Yangfan, Fei Kangqing, Xu Huilin, Gao Xiao‐Dong. Journal of the Science of Food and Agriculture, 2023, 103(1): 339-348.https://doi.org/10.1002/jsfa.12147

l A lipid scramblase TMEM41B is involved in the processing and transport of GPI-anchored proteins.Cao Shu-Ya, Liu Yi-Shi, Gao Xiao-Dong, Kinoshita Taroh, Fujita Morihisa. The Journal of Biochemistry, 2023, DOI:10.1093/jb/mvad041: mvad041.https://doi.org/10.1093/jb/mvad041

l Construction of a Yeast Cell-Based Assay System to Analyze SNAP25-Targeting Botulinum Neurotoxins.Chen Shilin, Li Feng, Liu Guoyu, Li Yuqing, Li Zijie, Liu Yishi, Nakanishi Hideki. Microorganisms, 2023, 11(5): 1125.https://doi.org/10.3390/microorganisms11051125

l Expression of a Siglec-Fc Protein and Its Characterization.Chi Kaijun, Xu Huilin, Li Hanjie, Yang Ganglong, Zhou Xiaoman, Gao Xiao-Dong. Biology, 2023, 12(4): 574.https://doi.org/10.3390/biology12040574

l Highly Efficient Synthesis of Rare Sugars from Glycerol in Endotoxin-Free ClearColi by Fermentation.Gao Yahui, Chen Zhou, Nakanishi Hideki, Li Zijie. Foods, 2023, 12(16): 3078.https://doi.org/10.3390/foods12163078

l Comprehensive in silico analysis of glycosylphosphatidylinositol-anchored protein (GPI-AP) related genes expression profiles in human normal and cancer tissues.Kong Wei-Ze, Liu Yi-Shi, Gao Xiao-Dong, Fujita Morihisa. Hereditas, 2023, 45(8): 669-683.https://doi.org/10.16288/j.yczz.23-043

l Lentinan-laden microspheres reprogram the tumor microenvironment and improve anti-PD-L1 efficacy.Wang Haixin, Gao Xiao-Dong, Yue Hua. Journal of Materials Chemistry B, 2023, 11(20): 4475-4484.https://doi.org/10.1039/d3tb00047h

l Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris.Wang Yasen, Wang Buqing, Gao Yahui, Nakanishi Hideki, Gao Xiao‐Dong, Li Zijie. Biotechnology Journal, 2023, DOI:10.1002/biot.202300259: 2300259.https://doi.org/10.1002/biot.202300259

l Rare sugar l-sorbose exerts antitumor activity by impairing glucose metabolism.Xu Hui-Lin, Zhou Xiaoman, Chen Shuai, Xu Si, Li Zijie, Nakanishi Hideki, Gao Xiao-Dong. Communications Biology, 2023, 6(1): 259.https://doi.org/10.1038/s42003-023-04638-z

l Studies on the Proteinaceous Structure Present on the Surface of the Saccharomyces cerevisiae Spore Wall.Yang Yan, Yang Ganglong, Li Zi-Jie, Liu Yi-Shi, Gao Xiao-Dong, Nakanishi Hideki. Journal of Fungi, 2023, 9(4): 392.https://doi.org/10.3390/jof9040392

2022

l Spore-Encapsulating Glycosyltransferase Catalysis Tandem Reactions: Facile Chemoenzymatic Synthesis of Complex Human Glycans.Chao Qiang, Li Tianlu, Jia Ji-Xiang, Li Zijie, Peng Peng, Gao Xiao-Dong, Wang Ning. ACS Catalysis, 2022, 12(5): 3181-3188.https://doi.org/10.1021/acscatal.1c05630

l Recent advances regarding the physiological functions and biosynthesis of D-allulose.Chen Zhou, Gao Xiao-Dong, Li Zijie. Frontiers in Microbiology, 2022, 13: 881037.https://doi.org/10.3389/fmicb.2022.881037

l Aloe Vera-Fermented Beverage Ameliorates Obesity and Gut Dysbiosis in High-Fat-Diet Mice.Fu Shijie, Dang Yanting, Xu Huilin, Li Aimin, Zhou Xiaoman, Gao Xiaodong, Li Zijie. Foods, 2022, 11(22): 3728.https://doi.org/10.3390/foods11223728

l Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores.Li Zijie, Li Wanjie, Wang Yasen, Chen Zhou, Nakanishi Hideki, Xu Xiangyang, Gao Xiao-Dong. Journal of Agricultural and Food Chemistry, 2022, 70(24): 7479-7489. https://pubs.acs.org/doi/10.1021/acs.jafc.2c01983

l Application of yeast spores as β-glucan particles.Liu Guoyu, Yang Yan, Tachikawa Hiroyuki, Gao Xiao-Dong, Nakanishi Hideki. Particuology, 2022, 71: 34-40.https://doi.org/10.1016/j.partic.2022.01.0131674-2001

l Triosephosphate Isomerase and Its Product Glyceraldehyde-3-Phosphate Are Involved in the Regulatory Mechanism That Suppresses Exit from the Quiescent State in Yeast Cells.Liu Guoyu, Yang Yan, Yang Ganglong, Duan Shenglin, Yuan Peng, Zhang Shuang, Li Feng, Gao Xiao-Dong, Nakanishi Hideki. Microbiology Spectrum, 2022, 10(4): e00897-00822. https://doi.org/10.1128/spectrum.00897-22

l An in vitro assay for enzymatic studies on human ALG13/14 heterodimeric UDP-N-acetylglucosamine transferase.Wang Chun-Di, Xu Si, Chen Shuai, Chen Zheng-Hui, Dean Neta, Wang Ning, Gao Xiao-Dong. Frontiers in Cell and Developmental Biology, 2022, 10: 1008078.https://doi.org/10.3389/fcell.2022.1008078

l Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.Wen Piaopiao, Chen Jingru, Zuo Chenyang, Gao Xiaodong, Fujita Morihisa, Yang Ganglong. Cells, 2022, 11(18): 2775.https://doi.org/10.3390/cells11182775

l Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.Xiang Meng-Hai, Xu Xin-Xin, Wang Chun-Di, Chen Shuai, Xu Si, Xu Xiang-Yang, Dean Neta, Wang Ning, Gao Xiao-Dong. Communications Biology, 2022, 5(1): 117.https://doi.org/10.1038/s42003-022-03066-9

l Receptor for advanced glycation end-products (RAGE) mediates phagocytosis in nonprofessional phagocytes.Yang Yan, Liu Guoyu, Li Feng, Carey Lucas B, Sun Changjin, Ling Kaiping, Tachikawa Hiroyuki, Fujita Morihisa, Gao Xiao-Dong, Nakanishi Hideki. Communications Biology, 2022, 5(1): 824.https://doi.org/10.1038/s42003-022-03791-1

 2021

l Global mapping of glycosylation pathways in human-derived cells. Huang Y F, Aoki K, Akase S, Ishihara M, Liu Y S, Yang G, Kizuka Y, Mizumoto S, Tiemeyer M, Gao X D, Aoki-Kinoshita K F, Fujita M. Dev Cell, 2021, 56(8): 1195-1209 e1197. https://doi.org/10.1016/j.devcel.2021.02.023

l A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins. Liu S S, Liu Y S, Guo X Y, Murakami Y, Yang G, Gao X D, Kinoshita T, Fujita M. Commun Biol, 2021, 4(1): 777.https://doi.org/10.1038/s42003-021-02337-1

l Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals. Yang J, Hirata T, Liu Y S, Guo X Y, Gao X D, Kinoshita T, Fujita M. FEBS Lett, 2021, 595(11): 1542-1558.https://doi.org/10.1002/1873-3468.14083

l Maca extracts regulate glucose and lipid metabolism in insulin-resistant HepG2 cells via the PI3K/AKT signalling pathway. Li A, Liu J, Ding F, Wu X, Pan C, Wang Q, Gao M, Duan S, Han X, Xia K, Liu S, Wu Y, Zhou Z, Zhang X, Gao X D. Food Sci Nutr, 2021, 9(6): 2894-2907.https://doi.org/10.1002/fsn3.2246

l Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles. Huang Y-F, Mizumoto S, Fujita M. Front Cell Dev Biol, 2021, 9.https://doi.org/10.3389/fcell.2021.709018

l Cell engineering for the production of hybrid-type N-glycans in HEK293 cells. Leng J X, Ren W W, Li Y, Yang G, Gao X D, Fujita M. J Biochem, 2021.https://doi.org/10.1093/jb/mvab051

Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system. Liu Y S, Matabaro E, Gao X D, Fujita M. J Biosci Bioeng, 2021, 131(3): 225-233.https://doi.org/10.1016/j.jbiosc.2020.10.004

l Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataea minuta. Yoko O T, Komatsuzaki A, Yoshihara E, Zhao S, Umemura M, Gao X D, Chiba Y. J Biosci Bioeng, 2021.https://doi.org/10.1016/j.jbiosc.2021.08.001

l Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT. Li F, Yang G, Tachikawa H, Shao K, Yang Y, Gao X D, Nakanishi H. J Gen Appl Microbiol, 2021, 67(1): 33-41.https://doi.org/10.2323/jgam.2020.04.002

l Functional analysis of the GPI transamidase complex by screening for amino acid mutations in each subunit. Liu S-S, Jin F, Liu Y-S, Murakami Y, Sugita Y, Kato T, Gao X-D, Kinoshita T, Hattori M, Fujita* M. Molecules, 2021:Accepted.

l Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension. Nakamura T S, Suda Y, Muneshige K, Fujieda Y, Okumura Y, Inoue I, Tanaka T, Takahashi T, Nakanishi H, Gao X D, Okada Y, Neiman A M, Tachikawa H. PLoS Genet, 2021, 17(8): e1009727.https://doi.org/10.1371/journal.pgen.1009727

l Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition. Guo X Y, Gao X D, Fujita M. PLoS One, 2021, 16(5): e0250805.https://doi.org/10.1371/journal.pone.0250805

2020

l Particulate Alum via Pickering Emulsion for an Enhanced COVID-19 Vaccine Adjuvant.Peng S, Cao F, Xia Y, Gao X-D, Dai L, Yan J, Ma G. Adv Mater.  2020 Oct;32(40):e2004210. https://doi.org/ 10.1002/adma.202004210.

l Chemo-enzymatic synthesis of the ALG1-CDG biomarker and evaluation of its immunogenicity. Jia JX, Kalisa NY, Lu TT, Zhou Z, Gao X-D*, Wang N*. Bioorg Med Chem Lett. 2020 Oct 17;30(24):127614. https://doi.org/10.1016/j.bmcl.2020.127614.

l Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system.Yi-Shi Liu, Emmanuel Matabaro, Xiao-Dong Gao, and Morihisa Fujita.J. Biosci. Bioeng., 2020 Nov 3;S1389-1723(20)30394-7. https://doi.org/10.1016/j.jbiosc.2020.10.004.

l Calnexin mediates the maturation of GPI-anchors through ER retention.Xin-Yu Guo, Yi-Shi Liu, Xiao-Dong Gao, Taroh Kinoshita, Morihisa Fujita.J. Biol. Chem., 2020 Sep 23;jbc.RA120.015577. https://doi.org/10.1074/jbc.RA120.015577.

l Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans. Chao Q, Ding Y, Chen ZH, Xiang MH, Wang N, Gao XD. Front Chem,2020  https://doi.org/10.3389/fchem.2020.00513

l Studies on the Properties of the Sporulation Specific Protein Dit1 and its Product Formyl Tyrosine. Basiony M, Yang Y, Liu G, Gao XD, Nakanishi H.J Fungi (Basel),2020 https://doi.org/10.3390/jof6020077

l Characteristics of SNARE proteins are defined by distinctive properties of SNARE motifs. Shao K, Li F, Yang Y, Wang N, Gao XD, Nakanishi H. Biochim Biophys Acta Gen Subj,2020 https://doi.org/10.1016/j.bbagen.2020.129658

l Characterization of alditol oxidase from Streptomyces coelicolor and its application in the production of rare sugars. Chen Z, Li Z, Li F, Wang N, Gao XD. Bioorg Med Chem,2020 https://doi.org/10.1016/j.bmc.2020.115464

l One-Pot Multienzyme Synthesis of Rare Ketoses from Glycerol. Li Z, Li F, Cai L, Chen Z, Qin L, Gao XD. J Agric Food Chem,2020 https://doi.org/10.1021/acs.jafc.9b06748

l Glycosylphosphatidylinositol Anchors. Liu S, Fujita M, Life Sciences 2020 https://doi.org/10.1016/B978-0-12-378630-2.00211-5

l Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast. cells Jin Y, Okamoto M, Chibana H, Liu G, Gao XD, Nakanishi H. Yeast,2020 https://doi.org/10.1002/yea.3502

l Cascade synthesis of rare ketoses by whole cells based on L-rhamnulose-1-phosphate aldolase. Chen Z, Li Z, Li F, Wang M, Wang N, Gao XD. Enzyme Microb Technol,2020 https://doi.org/10.1016/j.enzmictec.2019.109456

l MON2 Guides Wntless Transport to the Golgi through Recycling Endosomes. Zhao SB, Dean N, Gao XD, Fujita M. Cell Struct Funct,2020 https://doi.org/10.1247/csf.20012

l Encapsulation of Mannose-6-phosphate Isomerase in Yeast Spores and Its Application in l-Ribose Production. Li Z, Liu X, Nakanishi H, Gao XD. J Agric Food Chem,2020 https://doi.org/10.1021/acs.jafc.0c02399

l Cross-talks of glycosylphosphatidylinositol biosynthesis with glycosphingolipid biosynthesis and ER-associated degradation. Wang Y, Maeda Y, Liu YS, Takada Y, Ninomiya A, Hirata T, Fujita M, Murakami Y, Kinoshita T.Nat Commun,2020 https://doi.org/10.1038/s41467-020-14678-2

l Mammalian GPI-anchor modifications and the enzymes involved. Liu YS, Fujita M. Biochem Soc Trans,2020 https://doi.org/10.1042/BST20191142

2019

l Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans. Li ST, Lu TT, Xu XX, Ding Y, Li Z, Kitajima T, Dean N, Wang N, Gao XD. Nat Commun,2019  https://doi.org/10.1038/s41467-019-09752-3

l Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures. Ren WW, Jin ZC, Dong W, Kitajima T, Gao XD, Fujita M.J Biochem,2019 https://doi.org/10.1093/jb/mvz032

l Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao XD, Fujita M. Biochim Biophys Acta Gen Subj,2019 https://doi.org/10.1016/j.bbagen.2019.04.009

l Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins. Mensah EO, Guo XY, Gao XD, Fujita M. J Biosci Bioeng,2019 https://doi.org/10.1016/j.jbiosc.2019.04.005

l Origin identification of Chinese Maca using electronic nose coupled with GC-MS. Li A, Duan S, Dang Y, Zhang X, Xia K, Liu S, Han X, Wen J, Li Z, Wang X, Liu J, Yuan P, Gao XD. Sci Rep,2019 https://doi.org/10.1038/s41598-019-47571-0

l Production of l-Ribulose Using an Encapsulated l-Arabinose Isomerase in Yeast Spores. Liu X, Li Z, Chen Z, Wang N, Gao Y, Nakanishi H, Gao XD. J Agric Food Chem,2019 https://doi.org/10.1021/acs.jafc.9b00640

l Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin. Feng S, Zhang H, Xu S, Zhi C, Nakanishi H, Gao XD. Mater Sci Eng C Mater Biol Appl,2019  https://doi.org/10.1016/j.msec.2018.11.063

l Exploiting the Lymph-Node-Amplifying Effect for Potent Systemic and Gastrointestinal Immune Responses via Polymer/Lipid Nanoparticles. Du Y, Xia Y, Zou Y, Hu Y, Fu J, Wu J, Gao XD, Ma G.ACS Nano,2019 https://doi.org/10.1021/acsnano.9b04071

l Construction of functional chimeras of syntaxin-1A and its yeast orthologue, and their application to the yeast cell-based assay for botulinum neurotoxin serotype C. Shao K, Wang Q, Wang N, Gao XD, Nakanishi H. Biochim Biophys Acta Gen Subj,2019 https://doi.org/10.1016/j.bbagen.2019.07.005

l Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents Rao J, Zhang R, Liang H, Gao XD, Nakanishi H, Xu Y. Microb Cell Fact,2019  https://doi.org/10.1186/s12934-019-1137-6

l Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited. Wang Y, Hirata T, Maeda Y, Murakami Y, Fujita M, Kinoshita T.J Biol Chem,2019 https://doi.org/10.1074/jbc.RA119.007472

l Engineered disulfide bonds improve thermostability and activity of L-isoleucine hydroxylase for efficient 4-HIL production in Bacillus subtilis 168. Qiao Z, Xu M, Shao M, Zhao Y, Long M, Yang T, Zhang X, Yang S, Nakanishi H, Rao Z.Eng Life Sci,2019 https://doi.org/10.1002/elsc.201900090

2018

l N-Glycan-dependent protein folding and endoplasmic reticulum retention regulate GPI-anchor processing Liu YS, Guo XY, Hirata T, Rong Y, Motooka D, Kitajima T, Murakami Y, Gao XD, Nakamura S, Kinoshita T, Fujita M. J Cell Biol, 2018 https://doi.org/10.1083/jcb.201706135  

l Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation. Kitajima T, Xue W, Liu YS, Wang CD, Liu SS, Fujita M, Gao XD. FEBS J,2018 https://doi.org/10.1111/febs.14375

l pH-responsive charge-reversal polymer-functionalized boron nitride nanospheres for intracellular doxorubicin delivery. Feng S, Zhang H, Zhi C, Gao XD, Nakanishi H. Int J Nanomedicine,2018 https://doi.org/10.2147/IJN.S153476

l Alternative routes for synthesis of N-linked glycans by Alg2 mannosyltransferase. Li ST, Wang N, Xu XX, Fujita M, Nakanishi H, Kitajima T, Dean N, Gao XD.FASEB J,2018 https://doi.org/10.1096/fj.201701267R

l PER1, GUP1 and CWH43 of methylotrophic yeast Ogataea minuta are involved in cell wall integrity. Xu XX, Komatsuzaki A, Chiba Y, Gao XD, Yoko-O T. Yeast,2018 https://doi.org/10.1002/yea.3285

l Structural modeling and mutagenesis of endo-β-N-acetylglucosaminidase from Ogataea minuta identifies the importance of Trp295 for hydrolytic activity. Kitajima T, Jia Y, Komatsuzaki A, Cui J, Matsuzawa F, Aikawa SI, Gao XD, Chiba Y. J Biosci Bioeng,2018 https://doi.org/10.1016/j.jbiosc.2017.08.014

l Characterization of a yeast sporulation-specific P450 family protein, Dit2, using an in vitro assay to crosslink formyl tyrosine. Bemena LD, Mukama O, Wang N, Gao XD, Nakanishi H. J Biochem,2018 https://doi.org/10.1093/jb/mvx067

l Osw2 is required for proper assembly of glucan and/or mannan layers of the yeast spore wall. Pan HP, Wang N, Tachikawa H, Gao XD, Nakanishi H. J Biochem,2018 https://doi.org/10.1093/jb/mvx082

l PiggyBac-based screening identified BEM4 as a suppressor to rescue growth defects in och1-disrupted yeast cells. Mutumwinka D, Zhao SB, Liu YS, Mensah EO, Gao XD, Fujita M. Biosci Biotechnol Biochem,2018 https://doi.org/10.1080/09168451.2018.1482193

l Structural and functional analysis of Alg1 beta-1,4 mannosyltransferase reveals the physiological importance of its membrane topology. Xu XX, Li ST, Wang N, Kitajima T, Yoko-O T, Fujita M, Nakanishi H, Gao XD. Glycobiology,2018 https://doi.org/10.1093/glycob/cwy060

l Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose-type N-glycans. Jin ZC, Kitajima T, Dong W, Huang YF, Ren WW, Guan F, Chiba Y, Gao XD, Fujita M.J Biol Chem,2018 https://doi.org/10.1074/jbc.M117.813030

l Identification of a Golgi GPI-N-acetylgalactosamine transferase with tandem transmembrane regions in the catalytic domain. Hirata T, Mishra SK, Nakamura S, Saito K, Motooka D, Takada Y, Kanzawa N, Murakami Y, Maeda Y, Fujita M, Yamaguchi Y, Kinoshita T.Nat Commun,2018 https://doi.org/10.1038/s41467-017-02799-0

2017

l Nanodelivery systems for enhancing the immunostimulatory effect of CpG oligodeoxynucleotides. Zhang H, Gao XD. Mater Sci Eng C Mater Biol Appl,2017 https://doi.org/10.1016/j.msec.2016.03.045

l Production of encapsulated creatinase using yeast spores. Kong J, Li Z, Zhang H, Gao XD, Nakanishi H. Bioengineered,2017 https://doi.org/10.1080/21655979.2016.1241926

l Recent advances in the synthesis of rare sugars using DHAP-dependent aldolases. Li A, Cai L, Chen Z, Wang M, Wang N, Nakanishi H, Gao XD, Li Z. Carbohydr Res,2017 https://doi.org/10.1016/j.carres.2017.10.009

l Chitosan-Functionalized Graphene Oxide as a Potential Immunoadjuvant. Yan T, Zhang H, Huang D, Feng S, Fujita M, Gao XD. Nanomaterials (Basel),2017 https://doi.org/10.3390/nano7030059

l Consecutive hydrolysis of creatinine using creatininase and creatinase encapsulated in Saccharomyces cerevisiae spores. Kong J, Li Z, Zhang H, Gao XD, Nakanishi H. Biotechnol Lett,2017 https://doi.org/10.1007/s10529-016-2234-9  

l Quantitative study of yeast Alg1 beta-1, 4 mannosyltransferase activity, a key enzyme involved in protein N-glycosylation. Li ST, Wang N, Xu S, Yin J, Nakanishi H, Dean N, Gao XD. Biochim Biophys Acta Gen Subj,2017 https://doi.org/10.1016/j.bbagen.2016.09.023

l Graphene oxide-chitosan nanocomposites for intracellular delivery of immunostimulatory CpG oligodeoxynucleotides. Zhang H, Yan T, Xu S, Feng S, Huang D, Fujita M, Gao XD.Mater Sci Eng C Mater Biol Appl,2017 https://doi.org/10.1016/j.msec.2016.12.072

l Molecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins. Matabaro E, He Z, Liu YS, Zhang HJ, Gao XD, Fujita M. Sci Rep,2017 https://doi.org/10.1038/s41598-017-04330-3

l Yeast cells as an assay system for in vivo O-GlcNAc modification. Nakanishi H, Li F, Han B, Arai S, Gao XD. Biochim Biophys Acta Gen Subj,2017 https://doi.org/10.1016/j.bbagen.2017.03.002

l In vitro reconstitution of the yeast spore wall dityrosine layer discloses the mechanism of its assembly. Bemena LD, Mukama O, Neiman AM, Li Z, Gao XD, Nakanishi H.J Biol Chem,2017 https://doi.org/10.1074/jbc.M117.786202  

l β-1,6-glucan synthesis-associated genes are required for proper spore wall formation in Saccharomyces cerevisiae. Pan HP, Wang N, Tachikawa H, Nakanishi H, Gao XD.Yeast,2017 https://doi.org/10.1002/yea.3244

l Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. Nakamura TS, Numajiri Y, Okumura Y, Hidaka J, Tanaka T, Inoue I, Suda Y, Takahashi T, Nakanishi H, Gao XD, Neiman AM, Tachikawa H. Mol Biol Cell,2017 https://doi.org/10.1091/mbc.e17-08-0521

l Crystallographic analysis of murine p24γ2 Golgi dynamics domain. Nagae M, Liebschner D, Yamada Y, Morita-Matsumoto K, Matsugaki N, Senda T, Fujita M, Kinoshita T, Yamaguchi Y.Proteins,2017 https://doi.org/10.1002/prot.25242

2016

l Folate-conjugated boron nitride nanospheres for targeted delivery of anticancer drugs. Feng S, Zhang H, Yan T, Huang D, Zhi C, Nakanishi H, Gao XD. Int J Nanomedicine,2016 https://doi.org/10.2147/IJN.S110689

l Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae. Xu S, Zhang GY, Zhang H, Kitajima T, Nakanishi H, Gao XD. Microb Cell Fact,2016 https://doi.org/10.1186/s12934-016-0575-7

l Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling. Kinoshita T, Fujita M.J Lipid Res,2016 https://doi.org/10.1194/jlr.R063313

l 3D Structure and Interaction of p24β and p24 δGolgi Dynamics Domains: Implication for p24 Complex Formation and Cargo Transport. Nagae M, Hirata T, Morita-Matsumoto K, Theiler R, Fujita M, Kinoshita T, Yamaguchi Y.J Mol Biol,2016 https://doi.org/10.1016/j.jmb.2016.08.023

l A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO. Lee GH, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, Murakami KI, Kajikawa E, Takada Y, Saito K, Ikawa M, Hamada H, Maeda Y, Kinoshita T.J Cell Biol,2016 https://doi.org/10.1083/jcb.201605121