Zijie Li 李子杰

Associate Professor

Email: lizijie@jiangnan.edu.cn

Education & Experience

2012.7 – present Associate Professor, Jiangnan Universtiy

2011.9 – 2012.2 Joint Ph.D program, Georgia State University, USA

2010.3 – 2011.8 Joint Ph.D program, The Ohio State University, USA

2007.9–2012.6 Ph.D., National Glycoengineering Research Center, Shandong University

2003.9–2007.8 B.Sc., Hebei University of Science and Technology

Research Fields

(1) In vitro synthesis of functional sugars using free and immobilized enzymes.

(2) Production of high value added carbohydrates by microbial metabolic engineering.

(3) The biological activities of functional sugars and their mechanisms.

Selected Publications

1. Zijie Li, Xiaoxiao Liu, Hideki Nakanishi, Xiao-Dong Gao*. Encapsulation of mannose-6-phosphate isomerase in yeast spores and its application in L-ribose production. Journal of Agriculture and Food Chemistry. 2020, 68(25), 6892-6899.

2. Zijie Li*, Fen Li, Li Cai, Zhou Chen, Ling Qin, Xiao-Dong Gao*. One-pot multienzyme synthesis of rare ketoses from glycerol. Journal of Agriculture and Food Chemistry. 2020, 68(5), 1347-1353.

3. Zhou Chen, Zijie Li*, Fen Li, Mayan Wang, Ning Wang, Xiao-Dong Gao*. Cascade synthesis of rare ketoses by whole cells based on L-rhamnulose-1-phosphate aldolase. Enzyme and Microbial Technology. 2020,133:109456.

4. Zhou Chen, Zijie Li*, Fen Li, Ning Wang, Xiao-Dong Gao*. Characterization of alditol oxidase from Streptomyces coelicolor and its application in the production of rare sugars. Bioorganic & MedicinalChemistry. 2020, 28 (10), 115464.

5. Xiaoxiao Liu#, Zijie Li#, Zhou Chen, Ning Wang, Yahui Gao, Hideki Nakanishi*, Xiao-Dong Gao*. Production of L-ribulose using an encapsulated L-arabinose isomerase in yeast spores. Journal of Agriculture and Food Chemistry. 2019, 67(17), 4868-4875. (#equal contribution).

6. Shengtao Li, Tiantian Lu, Xinxin Xu, Yi Ding, Zijie Li, Toshihiko Kitajima, Neta Dean, Ning Wang*, Xiao-Dong Gao*. Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans. Nature Communications. 2019, 10(1):1813

7. Leo D Bemena, Omar Mukama, Aaron M Neiman, Zijie Li, Xiao-Dong Gao*, Hideki Nakanishi*In vitro reconstitution of the yeast spore wall dityrosine layer discloses the mechanism of its assembly. Journal of Biological Chemistry.2017, 292, 15880-15891

8. Aimin Li, Li Cai, Zhou Chen, Mayan Wang, Ning Wang, Hideki Nakanishi, Xiao-Dong Gao*, Zijie Li*. Recent advances in the synthesis of rare sugars using DHAP-dependent aldolases. Carbohydrate Research. 2017, 452, 108-115.

9. Zijie Li, Xiao-Dong Gao, Li Cai*. Synthesis of Rare Pentoses Using Microbial and Enzymatic Reactions. Current Organic Chemistry. 2016, 20, 1456-1464.

10. Mohui Wei#, Zijie Li#, Tiehai Li, Baolin Wu, Yunpeng Liu, Jingyao Qu, Xu Li, Lei Li, Li Cai, Peng George Wang*. Transforming Flask Reaction into Cell-Based Synthesis: Production of Polyhydroxylated Molecules via EngineeredEscherichia coli. ACS Catalysis. 2015, 5, 40604065. (#equal contribution)

11. Zijie Li, Xiaoru Wu , Li Cai , Shenglin Duan , Jia Liu, Peng Yuan, Hideki Nakanishi*, Xiao-Dong  Gao*. Enzymatic Synthesis of Rare Sugars with L-Rhamnulose-1-phosphate Aldolase from Thermotoga maritima MSB8. Bioorganic Medicinal Chemistry Letters, 2015, 25, 3980-3983.

12. Zijie Li, Yi Li, Shenglin Duan, Jia Liu, Peng Yuan, Hideki Nakanishi*, Xiao-Dong Gao*. Bioconversion of D-Glucose to D-Psicose with Immobilized D-Xylose Isomerase and D-Psicose 3-epimerase on Saccharomyces cerevisiae Spores. Journal of Industrial Microbiology and Biotechnology, 2015, 42, 1117-1128.

13. Zijie Li, Yingxin Qiao, Li Cai, Hideki Nakanishi*, Xiao-Dong Gao*. Characterization of Glycerol Phosphate Oxidase from Streptococcus pneumoniae and Its Application for Ketose Synthesis. Bioorganic Medicinal Chemistry Letter, 2015, 25, 504-507.

14. Zijie Li, Beibei He, Yahui Gao, Li Cai*. Synthesis of D-Sorbose and D-Psicose by Recombinant Escherichia coli. Journal of Carbohydrate Chemistry, 2015, 34(6), 349-357.

15. Libing Shi, Zijie Li, Hiroyuki Tachikawa, Xiao-Dong Gao*, Hideki Nakanishi*. Use of Yeast Spores for Microencapsulation of Enzymes. Applied and Environmental Microbiology. 2014, 80 (15): 4502-4510

16. Zijie Li, Yahui Gao, Hideki Nakanishi, Xiao-Dong Gao*, Li Cai*. Biosynthesis of Rare Hexoses Using Microorganisms and Related Enzymes. Beilstein Journal of Organic Chemistry. 2013, 9, 2434-2445.

17. Zijie Li, Li Cai*, Mohui Wei, Peng George Wang*. One-Pot Four-Enzyme Synthesis of Ketoses with class I D-Fructose 1,6-bisphosphate Aldolase from Staphylococcus carnosus and Rabbit Muscle. Carbohydrate Research. 2012, 357, 143-146.

18. Zijie Li, Li Cai*, Qingsheng Qi, Peng George Wang*. Enzymatic Synthesis of D-Sorbose and D-Psicose with Aldolase RhaD: Effect of Acceptor Configuration on Enzyme Stereoselectivity.Bioorganic Medicinal Chemistry Letter, 2011, 21, 7081-7084.

19. Zijie Li, Li Cai, Qingsheng Qi, Thomas J Styslinger, Guohui Zhao, Peng George Wang*. Synthesis of Rare Sugars with L-fuculose-1-phosphate Aldolase (FucA) from Thermus thermophilus HB8. Bioorganic Medicinal Chemistry Letter, 2011, 21, 5084-5087.