Hideki NAKANISHI 中西秀树
Jiangnan University
Email: hidekinakanishi@hotmail.com
Keywords: Yeast Sporulation, Spore Application, Saccharomyces cerevisiae Humanization; O-GlcNAcylation

Biography
He obtained his PhD degree from Tokyo University of Agriculture and Technology in 2001 (advisor: Dr. Yoshifumi Jigami). 2001-2006, Postdoctoral fellow in Dr Aaron Neiman’s laboratory at Stony Brook University. 2006-2007, Postdoctoral fellow, National Institute of Advanced Industrial Science and Technology (Dr. Shin-ichiro Nishimura’s Laboratory). 2007-2009, Postdoctoral fellow, Fukushima Medical University (Dr. Ikuo Wada’s Laboratory). 2009-2011, Assistant professor, Fukushima Medical University. He works as a professor at Jiangnan University in China since 2011. His research interest mainly focus on yeast spores.

Publications

1. Shao K, Li F, Yang Y, Wang N, Gao X-D, Nakanishi H. Characteristics of SNARE proteins are defined by distinctive properties of SNARE motifs. (2020) Biochim Biophys Acta. 1864. Online ahead of print.

2. Li Z, Liu X, Nakanishi H, Gao X-D. Encapsulation of mannose-6-phosphate isomerase in yeast spores and its application in l-ribose production. (2020) J Agric Food Chem. 68(25):6892-6899.

3. Basiony M, Yang Y, Liu G, Gao X-D, Nakanishi H.J. Studies on the Properties of the Sporulation Specific Protein Dit1 and its Product Formyl Tyrosine. (2020) J Fungi. 6: E77.

4. Jin Y, Okamoto M, Chibana H, Liu G, Gao X-D, Nakanishi H. Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells. (2020) Yeast. 37:359-371.

5. Li F, Yang G, Tachikawa H, Shao K, Yang Y, Gao X-D, Nakanishi H. Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT. (2020) J Gen Appl Microbiol. In press

6. Rao J, Zhang R, Liang H, Gao X-D, Nakanishi H, Xu Y. Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents. (2019) Microb Cell Fact. 18: 18.

7. Liu X, Li Z, Chen Z, Wang N, Gao Y, Nakanishi H, Gao X-D. Production of L-Ribulose Using an Encapsulated L-Arabinose Isomerase in Yeast Spores. (2019) J Agric Food Chem. 67: 4868-4875.

8. Shao K, Wang Q, Wang N, Gao X-D, Nakanishi H. Construction of functional chimeras of syntaxin-1A and its yeast orthologue, and their application to the yeast cell-based assay for botulinum neurotoxin serotype C. (2019) Biochimica et Biophysica Acta. 1863: 0-UNSP129396.

9. Feng S, Zhang H, Zhi C, Gao X-D, Nakanishi H. pH-responsive charge-reversal polymer-functionalized boron nitride nanospheres for intracellular doxorubicin delivery. (2018) Int J Nanomedicine. 13: 641-652.

10. Pan H-P, Wang N, Tachikawa H, Gao X-D, Nakanishi H. Osw2 is required for proper assembly of glucan and/or mannan layers of the yeast spore wall. (2018) J Biochem. 163: 293-304.

11. Bemena LD, Mukama O, Wang N, Gao X-D, Nakanishi H. Characterization of a yeast sporulation-specific P450 family protein, Dit2, using an in vitro assay to crosslink formyl tyrosine. (2018) J Biochem. 163: 123-131.

12. Bemena LD, Mukama O, Neiman AM, Li Z, Gao X-D, Nakanishi H. In vitro reconstitution of the yeast spore wall dityrosine layer to disclose the mechanism of its assembly. (2017) J Biol Chem. 292: 15880-15891.

13. Nakanishi H, Li F, Han B, Arai S, Gao X-D. Yeast cells as an assay system for in vivo O-GlcNAc modification. (2017) Biochim Biophys Acta. 1861: 1159-1167.

14. Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Production of encapsulated creatinase using yeast spores. (2017) Bioengineered. 8: 411-419.

15. Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Consecutive hydrolysis of creatinine using creatininase and creatinase encapsulated in Saccharomyces cerevisiae spores. (2017) Biotechnology Letters. 39: 261-267.

Graduate students
Doctors: Huaping Pan, Jun Kong, Bemena LD, Shini Feng, Feng Li, Kankai Shao
Masters: Libing Shi, Liping Wang, Mohamed LA, Baoxian Han, Yi Li, Mukama Omar, Zheng Yin, Dandi Huang, Caihong Wang, Xiaowen Wang, Yifan Jin, Xiaoxiao Liu, Basiony Mostafa.